Selective Chemokine Receptor Usage by Central Nervous System Myeloid Cells in CCR2-Red Fluorescent Protein Knock-In Mice (original) (raw)
Figure 6
Analysis of monocyte subsets in brain lesions of EAE Ccr2RFP/+Cx3cr1GFP/+ mice at peak EAE.
Confocal images of the periventricular lesions were obtained after staining with the 7/4 antibody. Images represent the same lesion (A) Merged images of red/CCR2, green/CX3CR1, and blue/7/4. (B) Red/CCR2 and green/CX3CR1. (C) Blue/7/4 and green/CX3CR1. (D) Red/CCR2 and blue/7/4. The majority of CCR2+ cells are 7/4+ and CX3CR1lo or negative, suggesting that they are classical infiltrating monocytes (see individual cell “1”). Ly6Clo monocytes (CX3CR1hi and CCR2) are also present (cell “2”), as well as T cells (“3”) and activated microglial cells (“4”).