Expression and Regulation of Cyclic Nucleotide Phosphodiesterases in Human and Rat Pancreatic Islets (original) (raw)
Figure 5
Activity and expression of PDEs in human pancreatic islets.
A. Human islets were homogenized and total PDE, PDE1, PDE3 and PDE4 activity was measured (n = 3). B. 20–25 µg of islet homogenates from three different human donors were run on SDS-PAGE and transferred to nitrocellulose membranes. Membranes were subjected to immunoblotting using PDE-isoform selective antibodies (one representative experiment shown).