Estradiol and Progesterone Regulate the Migration of Mast Cells from the Periphery to the Uterus and Induce Their Maturation and Degranulation (original) (raw)
Figure 5
Estradiol and progesterone treatment in vitro as well as their combined application up-regulate the expression of human tryptase in HMC-1 cells.
Human mast cell tryptase expression on HMC-1 cells treated for 1 h either with estradiol (E2; A), progesterone (P4; B) or (E2 + P4; C) was analyzed by Western Blot using GAPDH as house keeping gene. Physiological concentrations of both estradiol (A) and progesterone (B) corresponding to their concentrations towards middle menstrual cycle or just after ovulation takes place respectively as well as the combination of both hormones at their most effective concentration, significantly up-regulated the expression of human tryptase on HMC-1 cells (C). Data are expressed as Tryptase/GAPDH ratio (mean ± S.E.M.) *:p<0.05 as analyzed by ANOVA test followed by Tukey's test (A–B) or The Kruskall-Wallis test followed by Mann-Whitney-U test (C). Each experiment was done in triplicate; the blots are representative from three independent experiments. Tryptase expression in HMC-1 cells after hormonal treatment was compared to the controls in each case.