Cyclodextrin Induces Calcium-Dependent Lysosomal Exocytosis (original) (raw)
Figure 5
Secretion of the lysosomal enzyme β-hexosaminidase by HPB-CD-treated cells.
(A) Wt (circles) and NPC1 (squares) cells were incubated with (closed shapes) or without (open shapes) 2% HPB-CD. At the indicated times, an aliquot of media was assayed for β-hexosaminidase activity. (B) Wt cells were incubated with 2% HPB-CD in the presence (closed shapes) or absence (open shapes) of calcium and an aliquot of media was assayed for β-hexosaminidase activity. (C) Wt cells were incubated with 1 µM ionomycin for 20 min in the presence (closed shapes) or absence (open shapes) of calcium and an aliquot of media was assayed for β-hexosaminidase activity. All enzyme activities are expressed as the percentage of the total enzyme activity found in media and cells. * and ** denote statistically significant differences between treated and untreated cells with P<0.01 and P<0.05, respectively.