Resveratrol Enhances Antitumor Activity of TRAIL in Prostate Cancer Xenografts through Activation of FOXO Transcription Factor (original) (raw)
Figure 7
Activation of transcription factor FKHRL1 by resveratrol.
(A), Inhibition of FKHRL1 phosphorylation by resveratrol. Western blot analysis was performed to measure the expression of phospho-FKHRL1 in tumor tissues derived from control and/or drug treated mice on week 6. (B), Immunohistochemical examination of phospho-FKHRL1. Immunohistochemistry was performed to measure the expression of phospho-FKHRL1 in tumor tissues derived from control, resveratrol and/or TRAIL treated mice on week 6. (C), Quantification of FKHRL-positive cells in tumor tissues. (D), FKHRL1-DNA binding activity. Nuclear extracts were prepared from tumor tissues derived from control and drug-treated mice. Gelshift assay was performed to measure the FKHRL1-DNA binding activity as described in Materials and Methods.