Surface Expressed Nucleolin Is Constantly Induced in Tumor Cells to Mediate Calcium-Dependent Ligand Internalization (original) (raw)
Figure 9
Calcium dependent internalization of surface nucleolin.
DMEM-cultured HeLa cells were incubated at 37°C with mAb D3 (15 µg/ml) in the absence (A, D) or presence of additional 3 mM CaCl2 (B, E) or 5 mM EGTA (C, F). After 3 hours, cells were further incubated at room temperature for 90 min before PFA (7%) or PFA-Triton (7%-0.5%) fixation. The anti-nucleolin antibody mAb D3 was revealed by FITC-labeled goat anti-mouse antibodies. A scan corresponding to a cross-section toward the middle of the cell monolayer is shown.