Drug-Driven AMPA Receptor Redistribution Mimicked by Selective Dopamine Neuron Stimulation (original) (raw)
Figure 2
Addictive drugs cause rectification via AMPAR redistribution.
(A) Representative traces of AMPAR excitatory postsynaptic currents recorded at −70, 0 and +40 mV. Examples are shown from recordings 24 h post injection. (B) Individual and averaged normalized rectification indices (RIs) (mean ± s.e.m) of saline and each drug treatment. RIs of morphine (2.12±0.27), nicotine (2.06±0.23) and cocaine (1.72±0.14) groups were significantly different from the saline (1.12±0.08) control group (F(3,35) = 4.93, p<0.01, ANOVA. n = 7–15). (C) Representative electron micrographs of VTA sections from saline- or drug-treated animals. Large profiles (arrows) represent tyrosine hydroxylase (TH) immunoreactivity in dendrites (Den) forming asymmetrical synapses with boutons (b), and small profiles (arrowheads) represent GluA2 immunoreactivity. (D) Number of small profiles plotted against the distance from the postsynaptic density. (E) Same as in (C) but staining against PSD 95. (F) Same quantification as in (D) but for PSD 95.