Phosphatidylserine Increases IKBKAP Levels in Familial Dysautonomia Cells (original) (raw)
Figure 4
Validation of gene expression microarray analysis by QPCR.
FDB cells were treated with 100 µg/ml PS. RNA was extracted 24 hr following the addition of the supplement. A portion of each RNA sample was used for microarray analysis, and an aliquot was saved for experimental validation. QPCR analysis of genes shown by the gene expression microarray analysis to be (A) up-regulated or (B) down-regulated as a result of PS treatment. Each panel shows the levels in FDB cells after PS treatment relative to untreated FDB cells. (C) All values were normalized to a control, the LZIC gene transcript, that did not change as a result of PS treatment. QPCR experiments were amplified in triplicate; results shown are mean values ±S.D.