ReCLIP (Reversible Cross-Link Immuno-Precipitation): An Efficient Method for Interrogation of Labile Protein Complexes (original) (raw)
Figure 1
Titration of intracellular cross-linking of p120 and E-cadherin.
Western blot analysis of E-cadherin (top panels) and p120 (bottom panels) in whole cell lysates of A431 cells treated with the indicated concentrations of DSP (a, b) or DTME (c, d) between 0.01 mM and 1.0 mM. Samples were prepared under non-reducing (a, c) and reducing conditions (b, d) as indicated. Arrowheads indicate monomeric E-cadherin and p120, large cross-linked species are indicated with arrows, smaller crosslinked E-cadherin species are indicated with a diamond (⧫), and a possible cysteine-induced E-cadherin dimer is indicated with an asterisk (*). The chemical structures of DSP (e) and DTME (f) are shown, images were constructed with the DrawIt application in KnowItAll Informatics System v. 4.1 (Bio-Rad).