Murine Cytomegalovirus Infection of Neural Stem Cells Alters Neurogenesis in the Developing Brain (original) (raw)
Figure 1
CD133(+) cells are infected with MCMV in the developing brain.
A. Day-old neonates from timed breeders were infected intra-cerebrally (i.c.) either with 200 TCID50 of virulent, salivary gland passed recombinant MCMV expressing green fluorescent protein (GFP) or with mock inoculum, representing controls. A single cell suspension was prepared from the harvested brains by papain enzyme digestion at 7 d p.i. One million cells from both infected and control brains were stained for CD133, and analyzed by flow cytometry. B. Representative histogram showing MCMV infected CD133(+) cells, the black line represents control and the green line was obtained from MCMV-infected brain. Data shown are representative of three independent experiments using cells prepared from P7 brains obtained from 4, 3 and 5 neonates, respectively.