Resident Memory T Cells (TRM) Are Abundant in Human Lung: Diversity, Function, and Antigen Specificity (original) (raw)
Figure 1
Human lung contains large numbers of T cells.
(A) Non-inflamed normal lung tissue was obtained and stained for hematoxylin and eosin (H&E), CD3 (200×), CD4 and CD8 (400×). A representative experiment is shown, and three additional donors produced similar results. (B) T cells were counted in sections 12 µm thick and 500 µm wide and estimated the numbers of T cells in 1 mm3. (C) After 3-day of explant culture, lung-T cells were harvested and counted as described in Materials & Methods. Data is shown as Mean ± SEM of 10 experiments. (D) Lung-T cells were extracted from same specimen by conventional as well as lung explant method and counted as described in Materials and Method section. Data is shown as Mean ± SEM of 3 experiments. (E) Lung-T cells, Blood T cells from freshly isolated PBMCs (negative control), or KG1a cells (positive control) were stained for Ki67 and analyzed by flow cytometry.