Effect of Population Heterogenization on the Reproducibility of Mouse Behavior: A Multi-Laboratory Study (original) (raw)
Figure 8
Between-experiment variation versus within-experiment variation.
To assess the relative weight of between-laboratory variation versus within-laboratory variation, an F-ratio was calculated that reflects the partitioning of the ‘strain-by-block’ variance between all 24 blocks of one experimental design into variance due to variation between laboratories and variance due to variation within laboratories. For this, the mean squares of the ‘strain-by-laboratory’ interaction term were divided by the mean squares of the ‘strain-by-block’ interaction term. Data are displayed as mean F-ratios (+ s.e.m.; square-root-transformed) across all 29 behavioral measures for both conditions. F-ratios were significantly smaller in the heterogenized design (F1,28 = 4.678, p = 0.039), demonstrating that heterogenization increased within-experiment variation relative to between-experiment variation.