A Quantitative Comparison of Cell-Type-Specific Microarray Gene Expression Profiling Methods in the Mouse Brain (original) (raw)
Figure 2
Cell purification methods show differential susceptibility to contamination.
(A) GABA contamination indices of non-GABAergic cell types, (B) astrocyte contamination indices of non-astrocyte cell types, and (C) oligodendrocyte contamination indices of non-oligodendrocyte cell types. For an explanation of how the contamination indices were computed, see the Methods. (D) Mean sample contamination indices of each purification method for the three different categories of contaminants (from A, B, and C). Differences in mean contamination indices across methods were significant for each category of contaminant (ANOVA p<0.005). Asterisks indicate which means were significantly different from the lowest means (Tukey's post-hoc test, p<0.05), which were achieved by the Manual sorting method in the case of GABA and astrocyte contamination, and PAN in the case of oligodendrocyte contamination.