Maternal High Fat Diet Is Associated with Decreased Plasma n–3 Fatty Acids and Fetal Hepatic Apoptosis in Nonhuman Primates (original) (raw)
Figure 4
Maternal breast milk insulin and protein.
Analysis of insulin (A) and total protein levels (B) in breast milk from macaque dams in CTR (white bars) and HFD (black bars) maternal diet groups. A. Insulin was assayed by a commercially available primate RIA kit. HFD dams have significantly higher levels of insulin in their breast milk than CTR dams ( CTR; n = 11, HFD; n = 17, **P<.01 versus CTR, Wilcoxon rank sum test). B. Macaque breast milk total protein levels were measured from the aqueous layer using a BCA™ Protein Assay kit across CTR (white bars) and HFD (black bars) maternal diet groups. HFD breast milk contains significantly lower levels of total protein when compared to CTR ( CTR; n = 13, HFD; n = 17, *P<.05 versus CTR, Student's t-test).