Identification of Anchor Genes during Kidney Development Defines Ontological Relationships, Molecular Subcompartments and Regulatory Pathways (original) (raw)
Figure 4
Expression of marker genes in the ureteric tree at 15.5dpc.
Three compartments were analysed for anchor genes in the ureteric tree; the ureteric tip including the uretric tree terminal branch (UT), the cortical collecting duct (CCD) and medullary collecting duct (MCD). A) Examples of expression patterns seen by SISH are shown for UT and MCD genes. The top panel shows kidney sections with the region enlarged shown below. Arrows in b,d = UT and f,h = MCD expression. The genes identified as potentially CCD-specific did not validate by SISH. SISH images and text-annotated expression patterns for all genes are available on the GUDMAP website (http://www.gudmap.org). B) Schematic of 15.5dpc kidney divided into nephrogenic zone (top), renal cortex, and medulla showing segmentation of the ureteric tree. *indicates the regions collected by LCM representing the UT compartment. The expression of each gene giving either a specific or enriched expression pattern was painted onto the schematic and is shown in C) UT genes D) MCD specific anchor genes and E) MCD enriched genes. Slco4c1 was identified as the only UT-specific anchor gene.