Adenylate Cyclase Toxin Promotes Internalisation of Integrins and Raft Components and Decreases Macrophage Adhesion Capacity (original) (raw)
Figure 6
ACT-induced decrease of macrophage adhesion.
Treatment with 35 nM ACT for 10 min reduces J774A.1 cell adhesion. The effect of the toxin on adhesion was partially reduced by pre-treatment with nifedipine (100 µM), a Ca2+ channel inhibitor, KT5720 (56 nM), a PKA inhibitor and with calpeptin (50 µM), an inhibitor of the protease calpain. All molecules were pre-incubated for 30 min with J774A.1 cells before the addition of ACT. Adhesion was measured as described in Materials and Methods; the data shown is the mean ± SEM of at least three independent experiments.