Mouse Embryonic Stem Cells Inhibit Murine Cytomegalovirus Infection through a Multi-Step Process (original) (raw)
Figure 9
Comparison of MCMV resistance in MEF, iPS, and ES cells.
(A) Results from immunocytochemical analyses visualizing MCMV IE1-positive cells (red) in the 3 cell cultures after infection with MCMV at an MOI of 10. (B) Flow cytometry was used to confirm that iPS and ES both had lower numbers of IE1-positive cells than MEF, when infected at MOIs of 1 (P<0.01) and 10 (P<0.001). At an MOI of 10, iPS cells showed higher expression than ES cells of IE1 (7.27% vs. 2.17%) (P<0.001). (C) Results from in situ hybridization showing that MCMV signaling in all 3 cultures was proportional to the amount of IE1-positive cell expression. All presented experiments were performed at least 3 times, and data are given as the mean±SD. ** P<0.01, *** P<0.001, _t-_test.