Novel Pathway of Adipogenesis through Cross-Talk between Adipose Tissue Macrophages, Adipose Stem Cells and Adipocytes: Evidence of Cell Plasticity (original) (raw)
Figure 1
Scheme of co-culture between adipocytes, and ATMs/ASCs isolated from adipose tissue.
Characterization of the isolated adipocytes and ATMs present in the ATM/ASC fraction. Isolated adipocytes and ATMs/ASCs were cultured separately for 24 hours to allow the cells to reach equilibrium and then were co-cultured for an additional 24 hours. Finally, the cells were separated and cultured for another 24 hours or 48 hours. (A) At the end of these periods, the culture media and the cells were subjected to different studies. (B) Nile Red co-labeled with DAPI (light blue) indicates the presence of mature adipocytes (x100). (C) Immunofluorescence (40X) of adipocytes cultured for 24 hours, cells are CD14 (−). (D) ATMs/ASCs after 3 days in culture, labeled for CD14 (200X), (E) these cells are Nile Red (-).