Novel Pathway of Adipogenesis through Cross-Talk between Adipose Tissue Macrophages, Adipose Stem Cells and Adipocytes: Evidence of Cell Plasticity (original) (raw)
Figure 3
Differentiation of adipose stem cells to preadipocytes/adipocytes.
Isolated adipocytes and ATMs/ASCs were cultured separately for 24 hours to allow the cells to reach equilibrium and then were co-cultured for an additional 24 hours. The cells were then separated and ATMs/ASCs were cultured for another 2 days in regular medium followed by an additional 7 days in either the same medium or adipogenic medium (A) ATMs/ASCs without co-culture were cultured in regular medium for 2 days followed by an additional culture of 7 days in the same medium; (B) ASCs/ATMs with co-culture were cultured in regular medium for 2 days followed by an additional culture for 7 days in regular medium. (C) Fold change in gene expression of C/EBPα and PPARγ of ASCs/ATMs with and without co-culture, cell culture conditions are the same as described above (see 3A–B); (D) ASCs/ATMs without co-culture were cultured in regular medium for 2 days followed by an additional culture for 7 days in adipogenic medium; (E) ASCs/ATMs with co-culture were cultured for 2 days in regular medium followed by additional culture for 7 days in adipogenic medium; (F) Fold change in gene expression of C/EBPα and PPARγ of ASCs/ATMs with and without co-culture, cell culture conditions are the same as described above (see 3D–E).