Effects of Dietary Supplementation of Carnosine on Mitochondrial Dysfunction, Amyloid Pathology, and Cognitive Deficits in 3xTg-AD Mice (original) (raw)
Figure 3
Carnosine supplementation reduces intraneuronal Aβ but not tau accumulation in the hippocampus of 3xTg-AD mice.
Immunohistochemistry was employed to detect deposits of intraneuronal Aβ (A–D) and h-tau (E–H) in brain slices from treated (n = 3) and untreated (n = 3) 3xTg-AD mice (left column: 5× magnification, scale bar 200 µm; right column: 40× magnification of the hippocampal CA1 subregion, scale bar 20 µm). Compared to untreated 3xTg-AD mice (A,B), immunohistochemical staining shows a strong decrease of intraneuronal Aβ immunoreactivity in the hippocampus of carnosine treated 3xTg-AD mice (C,D). (I) Quantification of intraneuronal Aβ load as shown in B and D. Untreated 3xTg-AD mice (E,F) show comparable intraneuronal h-tau deposits in the hippocampus compared to treated mice (G,H). (J) Quantification of h-tau levels as shown in F and H. Error bars are shown as mean (± SEM); (*) indicates p<0.05.