Effects of Dietary Supplementation of Carnosine on Mitochondrial Dysfunction, Amyloid Pathology, and Cognitive Deficits in 3xTg-AD Mice (original) (raw)
Figure 4
Carnosine supplementation rescues mitochondria deficits in 3xTg-AD mice.
BN-PAGE was employed to assess the activity of mitochondrial complexes I, II, and IV in isolated mitochondria obtained from the hippocampus and cerebral cortex of control (PS1KI), untreated, and carnosine-treated 3xTg-AD (n = 3 to 5) mice at 12–14 m.o.a. (A,B) When compared with age-matched untreated mice, activity of mitochondrial complexes I and IV are found decreased in the hippocampus of 3xTg-AD mice while carnosine supplementation completely prevents the deficits. (C,D) When comparing complex activities of mitochondria obtained from the cortex of 3xTg-AD vs age-matched control mice, complex I and IV are found strongly compromised and carnosine treatment rescued these deficits. Error bars are expressed as mean (± SEM); (*) indicates p<0.05; (**) indicates p<0.01.