Dopaminergic Neuronal Loss, Reduced Neurite Complexity and Autophagic Abnormalities in Transgenic Mice Expressing G2019S Mutant LRRK2 (original) (raw)
Figure 1
Generation of LRRK2 transgenic mice.
A, Schematic showing the CMVE-PDGFβ-LRRK2 transgene and the positions of familial PD mutations. PCR primers for 5′ (P1/P2) and 3′ (P3/P4) genotyping are indicated. B, Semi-quantitative RT-PCR analysis of human LRRK2 mRNA expression in 2–3 month LRRK2 transgenic lines. Mouse β-actin mRNA is used as a loading control. The absence (−) or presence (+) of RT enzyme in the reaction is indicated. C, Western blot analysis of soluble extracts from hemi-brains of 2–3 month LRRK2 transgenic mice (TG), non-transgenic mice (NTG) or LRRK2 knockout (KO) mice using LRRK2-specific antibodies, JH5514 (human/mouse) or human-specific NB300-267. β-tubulin is used a control for protein loading. Bar chart showing densitometric quantitation of total LRRK2 levels (JH5514 antibody) in each transgenic line. LRRK2 levels are normalized to β-tubulin levels and expressed as a percent of NTG mice.