The Rate of NF-κB Nuclear Translocation Is Regulated by PKA and A Kinase Interacting Protein 1 (original) (raw)
Figure 5
Identification of PKAc and p65 interaction sties on AKIP 1A by peptide array analysis.
A peptide overlay of AKIP 1A with the purified catalytic domain of PKAc and p65. Fifteen-residue peptides covering amino acids 1-210 of AKIP 1A staggered by five amino acids (A) or eighteen-residue peptides covering amino acids 1-210 of AKIP 1A staggered by one amino acid (B) were spotted onto a membrane and overlaid with recombinant PKAc (A) or in vitro translated p65 (B). Binding of PKAc and p65 binding were detected by Western blotting using antibodies to PKAc or p65. The sequence, shown above or below the data, highlights the residues required for the interaction.