The Rate of NF-κB Nuclear Translocation Is Regulated by PKA and A Kinase Interacting Protein 1 (original) (raw)
Figure 7
Knock down of endogenous PKAc accelerates nuclear accumulation of endogenous p65.
HeLa cells were transfected with mock siRNA (lanes 1–3) or PKAc siRNA (lanes 4–6) for 36 hours, serum starved for 12 hours, and then stimulated with TNFα for 0, 15, or 45 min. Samples were analyzed on 10% SDS-polyacrylamide gels and Western blotted with antibodies to p65, PKAc, and lamin B.