Transplantation of Neuronal-Primed Human Bone Marrow Mesenchymal Stem Cells in Hemiparkinsonian Rodents (original) (raw)
Figure 1
Growth factor-induced neuronal differentiation of hMSCs.
(A) Diagram of multiple-stage dopaminergic neuronal differentiation (MultiDA), single-stage neuronal differentiation (SingleND), and single-step dopaminergic neuronal differentiation (SingleDA) [32] methods. (B) Morphology of hMSCs during the MultiDA procedure. Images were captured at the end of each stage (1-week) of sequential growth factor treatment. Representative phase-contrast images depicting hMSCs: prior to treatment (Ctrl; d0); after S1 (d7), S2 (d14), S2# (d14), S3 (d21), S3# (d21), and S3(2#) (d21; S1 growth factors removed after S2) of differentiation (n = 3). (C) Morphology of hMSCs during the SingleND procedure. Representative phase-contrast images of hMSCs after: p1 (1 week), p2 (2 weeks), and p3 (3 weeks) (n = 3). (D) Morphology of hMSCs during the SingleDA procedure. Representative phase-contrast images of hMSCs: prior to treatment (Ctrl; d0), and d6 and d12 post-treatment (n = 3). Ctrl, control undifferentiated hMSCs; S, stage; #, growth factors from S1 included; ND, neuronal differentiation; p, passage; d, day. Scale bar: 100 µm.