Transplantation of Neuronal-Primed Human Bone Marrow Mesenchymal Stem Cells in Hemiparkinsonian Rodents (original) (raw)
Figure 7
Increasing glial accumulation at graft sites during the initial 3-weeks post-intracerebral transplantation in hemiparkinsonian rats.
Immunohistological analysis revealed the accumulation of GFAP-positive astrocytes (red) and Iba-1-positive microglia/macrophages (green) at graft sites (n = 3). Representative images depicting (A–C) neuronal-primed hMSC graft sites in the striatum, and (D–F) corresponding region controls in the contralateral hemisphere, at 1-day (left column), 7-days (middle column) and 21-days (right column). (A) Initially, at 1-day post-transplantation little GFAP or Iba-1 was detected. (B) By 7-days grafts were densely surrounded by a layer of GFAP-positive and Iba-1-positive cells, and contained an autofluorescent core (white arrow). (C) At the end of the 21-day period a marked astrogliosis was present in the graft region. Both striatal and nigral grafts exhibited this glial accumulation, which suggests the presence of an inflammatory response at graft sites. (D–F) This pattern of glial distribution was absent from the contralateral hemisphere. Images shown are maximum projection z-stacks. Nuclei have been counterstained with DAPI (blue). Scale bar: 150 µm.