Organometallic Iron(III)-Salophene Exerts Cytotoxic Properties in Neuroblastoma Cells via MAPK Activation and ROS Generation (original) (raw)
Figure 1
Comparative analysis of the cytotoxic effect of Fe-SP on NB and other cancer cell lines.
(A) Viability of NB cell lines upon Fe-SP treatment. The cytotoxic effect of Fe-SP (0–3 µM) on human NB cell lines (SK-N-SH, SH-SY5Y, SMS-KCNR) was compared to a human cancer cell line of different origin (PC-3) and endothelial cells (HUVEC). Treatment with SP served as a negative control. The MTS viability assay was carried out as described (Materials and Methods). Data are expressed as the mean of the triplicate determinations (X±SD) of a representative experiment in % cell viability of untreated cells [100%]. (B,C) Differential effect of Fe-SP on cell growth in a NCI60 cancer cell line screen. Fe-SP effects were screened in a NCI60 cell line growth assay (http://dtp.nci.nih.gov/screening.html). Cells were treated in 96 well plates and cell growth of the TCA fixed treated and untreated cells assessed after 48 h.