Coenzyme Q10 Reduces Ethanol-Induced Apoptosis in Corneal Fibroblasts (original) (raw)
Figure 2
Pretreatment of corneal fibroblasts for 2 h with 10 µM CoQ10 inhibited the cell apoptosis induced by EtOH exposure.
(A–D) Cells were pretreated with or without CoQ10, followed by different EtOH treatment (0.4%, 2%, 4%, and 20%) for 20 s. The apoptotic cells were stained with annexin V-PI for flow-cytometric analysis at 4, 8, and 12 h. (E) The bar diagram shows the comparison of the relative fluorescence of annexin V–FITC fluorescence intensity at different times. Data represent the results of three independent experiments (means ± SD; *P<0.05 compared with the control group; †P<0.05 compared with the EtOH group).