Passive Immunization Reduces Behavioral and Neuropathological Deficits in an Alpha-Synuclein Transgenic Model of Lewy Body Disease (original) (raw)
Figure 1
Plasma antibody titers and effects of passive immunization on motor behavior in passively immunized α-syn tg mice.
(A) Antibody titers determined by ELISA in non-tg or α-syn tg mice immunized with the C-terminal antibody (9E4) or IgG1 controls. Horizontal lines represent the mean of the data, whilst the points represent the spread of the individuals in each group. To examine the effects of immunization with the 9E4 antibody on motor behavior in the α-syn tg, mice were tested in the rotarod and pole tests. (B) Pole test performance (time taken to traverse pole) by non-tg mice or α-syn tg mice immunized with IgG1 or 9E4. N = 20 mice per group; 12 month old. Error bars represent mean ± SEM. (C) Rotarod performance (time spent on rotating rod) by non-tg mice or α-syn tg mice immunized with IgG1 or 9E4. Error bars represent mean ± SEM. When analyzing rotarod results (*) indicates p<0.05, when comparing α-syn tg immunized with IgG1 to non-tg group by repeated-measures two-way ANOVA and (#) indicates p<0.05, when comparing α-syn tg mice immunized with 9E4 to IgG1 immunized α-syn tg mice using repeated-measures two-way ANOVA. When analyzing pole test results (*) indicates p<0.05, when comparing α-syn tg immunized with IgG1 to non-tg group by one-way ANOVA with post hoc Dunnett's and (#) indicates p<0.05, when comparing α-syn tg immunized with 9E4 to IgG1 immunized α-syn tg mice by one-way ANOVA with post hoc Dunnett's.