Exogenous Expression of Human apoA-I Enhances Cardiac Differentiation of Pluripotent Stem Cells (original) (raw)
Figure 8
Effect of apoA-I gene transfer on the cardiac differentiation of IMR90 human IPSCs.
(A) Percentage of ESC-derived cardiomyocytes (α-actinin (sarcomeric)-positive cells) 30 days after plating as determined by flow cytometry. (B) The beating EBs were digested into monolayers and the cardiomyocytes were identified with immunnohistochemistry using antibody specific to the cardiac troponin-T. Data shown as mean ± SEM from at least 3 independent experiments, *p<0.05; **p<0.005. Scale bar = 50 µm.