The Role of miR-103 and miR-107 in Regulation of CDK5R1 Expression and in Cellular Migration (original) (raw)
Figure 1
Correlation between p35 and miRNAs expression in cell lines.
A) Expression of mature miR-195, miR-16, miR-15a, miR-107, miR-103 and miR-34a measured by Real-Time PCR on total RNA extracted from SK-N-BE, SH-SY5Y, HEK-293, DU-145 and MCF-7 cell lines. The expression levels are normalised on the expression of the endogenous control RNU6B. RNU6B levels were comparable in all samples tested. B) Western blot for p35 detection in protein extracts from the same cell lines. Expression is normalised on β-actin. A representative experiment is shown at the top of the panel. C) Correlation analysis between each miRNA and p35 protein levels in the five cell lines, r = Pearson's correlation coefficient.