The Role of miR-103 and miR-107 in Regulation of CDK5R1 Expression and in Cellular Migration (original) (raw)
Figure 2
p35 protein and CDK5R1 mRNA expression in pre-miR-103/107 and anti-miR-103/107 transfected and untransfected SK-N-BE cells.
A) Western blot analysis of p35 in the conditioned media of control SK-N-BE cells (lane 1 and 2), 50 nM and 100 nM pre-miR-103 and pre-miR-107 transfected cells, at 48 h from transfection. A representative experiment is shown at the top of the panel. (*p<0.05, **p<0.01). B) Western blot analysis of p35 in the conditioned media of control SK-N-BE cells (lane 1 and 2), 50 nM and 100 nM anti-miR-103 and anti-miR-107 transfected cells, at 48 h from transfection. A representative experiment is shown at the top of the panel. (*p<0.05). C) CDK5R1 mRNA expression level measured by qRT-PCR after 48 h from transfection with 100 nM pre-miR-107, pre-miR-103, anti-miR-107 and anti-miR-103 normalised on housekeeping GAPDH transcript. The level of GAPDH expression was comparable in all samples tested. (*p<0.05, **p<0.01).