The Role of miR-103 and miR-107 in Regulation of CDK5R1 Expression and in Cellular Migration (original) (raw)
Figure 4
Interaction between miR-103/107 and CDK5R1 3′-UTR.
The 2688 bp sequence of the CDK5R1 3′-UTR was cloned upstream the Renilla luciferase gene generating the pGL4.71P-UTR construct. This was cotransfected into SK-N-BE cells with a firefly luciferase report vector for luciferase normalization. Cells were treated with the transfection agent alone (Lipofectamine) or with 100 nM pre-miR-103 or pre-miR-107. Luciferase activity was assessed 48 h after transfection of pre-miRNAs (*p<0.01).