IL-17RA Is Required for CCL2 Expression, Macrophage Recruitment, and Emphysema in Response to Cigarette Smoke (original) (raw)
Figure 1
Cigarette smoke extract enhances in vitro Th17 polarization.
Naïve CD4+ or CD8+T cells from C57BL/6 spleen were activated by plate bound anti-CD3 and anti-CD28 in the presence of TGF-β, IL-6, anti-IFN-γ and anti-IL4. Where indicated, 0.5% CSE or 200nM FICZ were added into the culture. On day 3, cells were harvested and intracellular cytokines were analyzed by FACS (A, C). Tissue culture supernatants were harvested from in vitro polarized Th17 cells for secreted IL-17 by Luminex (C, n = 5 per group, * denotes p<0.05 compared to vehicle). RNA was extracted from the same cells for gene expressions analysis by real-time RT-PCR (D, n = 5 per group, * denotes p<0.05 compared to vehicle-HBSS).