IL-17RA Is Required for CCL2 Expression, Macrophage Recruitment, and Emphysema in Response to Cigarette Smoke (original) (raw)
Figure 2
Cigarette smoke extract enhances Th17 differentiation in vivo.
(A) OTII mice were given 50 µg OVA in 50 µL HBSS or CSE intratracheally on day 0, day 7 and sacrificed on day 14. Single cell suspensions harvested from lung digest and mediastinal lymph nodes were re-stimulated with PMA and ionomycin in the presence of Golgi-plug for 5h, intracellular cytokines were analyzed by FACS (A). Gene expression in lung tissue was analyzed by real-time RT-PCR (B, n = 5 per group, * denotes p<0.05 compared to OVA alone).