IL-17RA Is Required for CCL2 Expression, Macrophage Recruitment, and Emphysema in Response to Cigarette Smoke (original) (raw)
Figure 3
Requirement of AhR for enhanced in vitro Th17 polarization by cigarette smoke extract.
Naïve CD4+ T cells from Ahr−/− spleen were activated by plated bounded anti-CD3 and anti-CD28 in the presence of TGF-b, IL-6, anti-IFN-γ and anti-IL4. Where indicated, 0.5% CSE or 200 nM FICZ were added into the culture. On day 3, cells were harvested and intracellular cytokines were analyzed by FACS (A). Tissue culture supernatants were harvested from in vitro polarized Th17 cells from Ahr−/− spleen for secreted IL-17 by Luminex and IL-22 by ELISA (B, n = 5 per group, * denotes p<0.05 compared to vehicle-HBSS).