IL-17RA Is Required for CCL2 Expression, Macrophage Recruitment, and Emphysema in Response to Cigarette Smoke (original) (raw)
Figure 4
Th17 cytokines induce MMP expression in HBE cells.
(A) Primary HBE cells were stimulated with media or a combination of 10/-50 ng/ml IL-22 and 50 ng/ml of IL-17A for 48 hours (n = 3 donors per condition). RNA was harvested and subjected to hybridization on Hu-133 arrays for RNA sequencing as described in the Methods. Transcript abundance or hybridization intensity was measured on normalized data in Qseq software (Lasergene). Scatter plot analysis of normalized transcript abundance for Affymetrix (x-axis) and RNA-seq (y-axis) are shown. (B) Relative gene expression of MMPs and TIMP2 from RNA-seq data. (C) Up-regulation of Ccl2 by IL-17 and IL-22 combination treatment in primary HBE cells as measured by RNAseq. (D) CCL2, MMP3 and MMP12 gene expression were analyzed by real-time RT-PCR.