IL-17RA Is Required for CCL2 Expression, Macrophage Recruitment, and Emphysema in Response to Cigarette Smoke (original) (raw)
Figure 6
Requirement of IL-17RA for emphysema.
Representative histological sections of mouse lungs stained with hematoxylin and eosin after six months of exposure to air (sham exposed) (A,B) or cigarette smoke (C,D) demonstrate C57BL/6 mice develop alveolar enlargement (C) as opposed to IL-17RA−/− mice (D). (E) Enlargement of airspaces was quantified using the mean linear intercept method. This method determines the parenchymal surface area relative to lung volume. An increase in Lm represents an increase in the alveolar space as seen in the WT smoking mice versus sham air. This increase in Lm was not seen in the IL-17A−/− mice over sham air controls. All lung tissues were inflated to 25cm water pressure with neutral buffered formalin. Original magnification: x 50 of representative sections.