SCFAs Induce Mouse Neutrophil Chemotaxis through the GPR43 Receptor (original) (raw)
Figure 4
GPR43−/− neutrophils do not migrate in response to propionate or phenylacetamide, but display a normal response to fMLP.
Chemotaxis of wild type (WT) and GPR43−/− neutrophils was evaluated in a transwell migration assay (A) and EZ-Taxiscan Chamber (B–C). Chemotaxis of wildtype or GRR43−/− neutrophils in a transwell migration assay was measured to propionate (1 mM), phenylacetamide (p-acetamide, 10 µM) or fMLP (1 µM). Data show the mean ± S.E.M. (n = 3) number of migrating neutrophils normalized to the control condition (non-treated). *p<0.05 compared to wildtype cells (paired t-test) (A). Chemotaxis of wildtype or GRR43−/− neutrophils in an EZ-Taxiscan chamber was measured to propionate (25 mM), phenylacetamide (p-acetamide, 50 µM) or fMLP (3 µM). The mean ± S.E.M. (n = 3) % of neutrophils migrating (past a 50 µm horizon) *p<0.05 compared with control condition (paired t-test) (B) and centre-zeroed tracks and horizon plots, as described in figure 1, (C) are shown. The data in B–C are derived from 6–10 movies.