Decellularized Matrix from Tumorigenic Human Mesenchymal Stem Cells Promotes Neovascularization with Galectin-1 Dependent Endothelial Interaction (original) (raw)
Figure 4
Histological sections of MESA plugs after 7 days in vivo.
A: Photomicrograph of a MESA implant showing a 1 cm diameter matrigel plug with a centrally implanted sponge (arrow, “S”). B: Haemotoxylin and Eosin stain of a sponge loaded with ECBM-MV2 medium. C,D: Histological sections stained with murine specific anti-CD34 antibody visualized by brown chromogen diaminobenzidine. C: Field of view of matrigel adjacent to sponge seeded with -BC8 cells. D: Field of view of matrigel adjacent to sponge seeded with -BD11 cells. E: Chalkley count quantification of vasculature adjacent to the Matrigel® embedded sponge. *P<0.05, Kruskal-Wallis. F,G: Histological sections showing migratory cells within the Matrigel® surrounding F, control medium sponges or G–I: sponges with -BD11 decellularized matrix, stained blue with Masson's trichrome. H,I: Anti-CD34 antibody was used to visualize endothelial cells (brown) in regions of the Matrigel® I: with or H: without decellularized matrix. Scale bar, 100 µm.