Osmosensitivity of Transient Receptor Potential Vanilloid 1 Is Synergistically Enhanced by Distinct Activating Stimuli Such as Temperature and Protons (original) (raw)
Figure 2
HEK293-TRPV1 cells scarcely respond to hypotonic stimuli in a temperature-dependent fashion.
(A) Representative single cell traces of the fluorescence ratio during perfusion with a hypotonic solution (250 mOsm) at various temperatures. The temperature was maintained throughout the respective recording. The line on the top indicates the timing of the change in osmolality between 300 mOsm (gray) and 250 mOsm (black). (B) Summary of the change in the fluorescence ratio in HEK293-TRPV1 (filled bars) and HEK293 (open bars) cells. Data are differences between fluorescence ratios 2 min before and 4 min after the change of the solution. Values are the mean ± SEM. HEK293-TRPV1: n = 147 (24°C), n = 145 (30°C), n = 105 (36°C), n = 108 (40°C). HEK293: n = 111 (24°C), n = 102 (30°C), n = 112 (36°C), n = 108 (40°C).