Comparative Analysis of mRNA Isoform Expression in Cardiac Hypertrophy and Development Reveals Multiple Post-Transcriptional Regulatory Modules (original) (raw)
Figure 3
Comparison of mRNA isoform regulation in cardiac hypertrophy and development.
(A) Comparison of SI for skipped exons (SE). Skipped exons were defined by cDNAs/ESTs. Grey dots are all expressed SE events, and blue dots are the ones that are developmentally regulated and experimentally validated in a previous study [25]. The dotted red line is based on linear regression of blue dots. Pearson correlation coefficient was calculated for each plot, and its E value was estimated based on random sampling of the same number of exons (see Materials and Methods for detail). (B) Venn diagram showing the highly regulated SEs in hypertrophy and development. Highly regulated SEs were selected using 1*SD of SI. (C) and (D) Regulation of alternative first and last exons. U and D are upstream and downstream terminal exons, respectively. Alternative terminal exons were analyzed using difference in log2(U/D) or log2(D/U), where U/D or D/U are ratios of probe set intensities between U and D.