Uncoordinated Loss of Chromatid Cohesion Is a Common Outcome of Extended Metaphase Arrest (original) (raw)
Figure 6
Chromosome scattering is prevented by inhibition of the two pathways that remove cohesion.
(A) Selected images from time-lapse imaging sequences of Cyclin B Δ86 HeLa FRT cells expressing histone H2b:mRFP and subjected to the indicated perturbations. The experimental scheme is depicted on top. Time (in mins) is indicated on the lower right of each panel; time 0 is NEBD. Scale bar, 5 µm. (B and C) Quantification of the experiments in (A); (B) the percentage of total cells in mitosis that scatter for each condition and (C) the time that those cells remain in mitosis before scattering is plotted (only cells that scattered were analyzed for the double separase/Wapl inhibition); each mark represents one cell and the black line indicates the average.