Frequent Alteration of MLL3 Frameshift Mutations in Microsatellite Deficient Colorectal Cancer (original) (raw)
Figure 3
DNA methylation analysis using quantitative bisulfite pyrosequencing in primary CRCs and MLL3 relative expression, protein analysis in different CRC cell lines.
(A) Example of pyrogram results using CpG island (primer region for pyrosequencing was shown in Figure 1), with polymorphic position C/T highlighted. Sequence reads TC/TGTC/TGGAGGAGGATAAGAG, Pyrogram in left side shows.normal colon and primary CRC (Right side). (B) Result of relative expression in normal colon and colon cancer cell lines analyzed by qPCR for the full length transcript (Probe A: Hs01005501_m1) and a mixture of the full-length and truncated transcripts (Probe B and C: Hs01005520_m1, Hs01005539_m1). Relative expression in probe A was down-regulated in 5 out of 6 cell lines examined. And the two other probes (Probe B and C) demonstrate minimal down-regulation (or even up-regulation) in these cell lines.