Human Embryonic and Fetal Mesenchymal Stem Cells Differentiate toward Three Different Cardiac Lineages in Contrast to Their Adult Counterparts (original) (raw)
Figure 4
Analysis by qRT-PCR of expression of pluripotency and cardiac genes in hMSCs alone or after co-incubation with nrCMCs.
(A) hESC-MSCs expressed most cardiac-specific genes, which were significantly upregulated after co-incubation with nrCMCs. Expression of the cardiac progenitor genes, Islet-1 and c-kit, was downregulated in the presence of nrCMCs. (B–D) The fetal hMSC types expressed a variety of cardiac-specific genes, which were upregulated after co-culture with nrCMCs. Islet-1 and c-kit mRNA levels decreased in the presence of nrCMCs with the exception of the upregulation of c-kit gene expression in fetal BM MSCs following their co-culture with nrCMCs. (E–F) ANP, Cx43, VEGF and c-kit gene expression was detected in adult hMSCs before and after co-incubation with nrCMCs. (A–F) hMSCs did not express the pluripotency genes Oct-4 and Nanog. #P<0.05 vs specific hMSC monoculture; *P<0.01 vs specific hMSC monoculture; †P<0.001 vs specific hMSC monoculture.