Allopregnanolone Promotes Regeneration and Reduces β-Amyloid Burden in a Preclinical Model of Alzheimer's Disease (original) (raw)
Figure 7
Allopregnanolone treatment inhibited microglial activation.
Equal amounts of frontal-parietal-temporal cortex samples from 3xTgAD mice treated with APα (10 mg/kg) or vehicle were loaded onto the gel. Microglial activation was determined with anti-CD11b/c (OX42) antibody by immunoblot analysis. (A) APα treatment significantly decreased OX42 expression in both the pre-pathology treatment group (26.2±4.6%, P<0.05) and the post-pathology treatment group (18.0±7.4%, P = 0.05). Bars represent mean relative expression ± SEM; * _P_≤0.05. (B) Reduction in microglial staining by APα treatment in hippocampal CA1 region. Representative image of immunofluorescent staining with microglial marker anti-Iba1 in 3xTgAD brains indicated substantial reduction of reactive microglia in the hippocampal CA1 region in APα treated 3xTgAD mice relative to vehicle (Iba1-labeled microglia cells shown in red and DAPI-labeled nuclei shown in blue).