Allopregnanolone Promotes Regeneration and Reduces β-Amyloid Burden in a Preclinical Model of Alzheimer's Disease (original) (raw)
Figure 8
Allopregnanolone increased myelination in brain of 3xTgAD mice.
Equal amount of frontal-parietal-temporal cortex samples from 3xTgAD mice treated with APα (10 mg/kg) or vehicle were loaded onto the gel. The expression of CNPase was measured using anti-CNPase antibody by immunoblot analysis as an indicator of oligodendrocytes and myelination. (A) APα treatment significantly increased CNPase expression in the pre-pathology group in both nonTg (P<0.01) and 3xTgAD mice (P<0.05). No significant change in CNPase expression was observed in the post-pathology treatment group in nonTg mice between APα treatment and the vehicle control. However, in 3xTgAD mice, APα treatment significantly increased CNPase expression (P<0.05). Bars represent mean relative expression values of CNPase relative to β-actin ± SEM; * P<0.05 and ** P<0.01 compared with vehicle control group. (B–D) Region-specific enhancement of myelination was observed in APα-treated mice. Representative image of CNPase immunostaining showed greater immunoreactivity in the hippocampal CA1 (B), entorhinal cortex (C) and primary somatosensory cortex (D) regions of APα-treated 3xTgAD mice.