Cyclic AMP Control Measured in Two Compartments in HEK293 Cells: Phosphodiesterase KM Is More Important than Phosphodiesterase Localization (original) (raw)
Figure 5
Different PDEs dominate at different cAMP concentrations.
Average relative BRET response 21–30 min after addition of forskolin (A, B) or PGE1 (C, D) in combination with PDE inhibitors to HEK293 cells transfected with CAMYEL or PDE2-CAMYEL. Different concentrations of forskolin or PGE1 was added alone or in combination with 50 nM roflumilast or 400 nM cilostamide and 300 nM MP-10 as indicated. From curves similar to those shown in Figure 4, the change in BRET signal was calculated relative to the maximum response (10 µM forskolin plus all three PDE inhibitors). The results represent the mean ± SEM of three experiments. ns (not significant), P>0.05; *, 0.01<P<0.05; **, 0.001<P<0.01.