CaMKIIδB Mediates Aberrant NCX1 Expression and the Imbalance of NCX1/SERCA in Transverse Aortic Constriction-Induced Failing Heart (original) (raw)
Figure 5
Effect of calmodulin inhibition on TAC-induced cardiomyocyte apoptosis.
TUNEL staining was used to assess the number of apoptotic cells. Triple staining was performed: TUNEL (green), TO-PRO3 (blue), α-actinin (red). (A) Bar graph shows the increased percentage of TUNEL-positive apoptotic cardiomyocyte nuclei (green fluorescence) 8 weeks after TAC. Scale bar = 20 µm. (B) Quantification of TUNEL-positive apoptotic cardiomyocytes in TAC mice with or without DY-9836 (20 mg/kg) treatment. Data are expressed as percentage of sham-operated animals (mean ± SEM, n = 6). **P<0.01 vs. sham; ##P<0.01 vs. vehicle. (C) Confocal microscopic images of double staining with anti-active caspase-3 (green fluorescence) and anti-troponinT (red fluorescence) antibodies in the heart tissue, indicating that the predominant activation of caspase-3 occurred in TAC mice. The number of cells showing increases in active caspase-3 was significantly reduced by DY-9836 (20 mg/kg) treatment. DAPI was used as a counterstain, to label the cell nuclei (blue). Scale bar = 20 µm.