Transcriptional Activation of OsDERF1 in OsERF3 and OsAP2-39 Negatively Modulates Ethylene Synthesis and Drought Tolerance in Rice (original) (raw)
Figure 2
OsDERF1 contains a transcriptional activation domain.
Left panel shows the schematic diagrams of various constructs used for transactivation described in Materials and Methods. DNA-BD indicates GAL4 DNA-binding domain; GAL4 AD for GAL4 activation domain; CDS and numbers for coding sequence length of OsDERF1. Right panel shows the β-galactosidase colony-lift filter assay and the yeast growth at different diluted concentration of the colony.